Recombinant Glycerophosphodiester Phosphodiesterase 1 (GDE1)
甘油磷酸二酯磷酸二酯酶1(GDE1)重組蛋白
[ PROPERTIES ]
Article Number:XY94979RPHu
Specifications and prices: 50ug 1800.00 100ug 2880.00
Source: Prokaryotic expression
Host: E.coli
Residues:Ala40~Pro111
Tags: N-terminal His and GST Tag
Subcellular Location: Nucleus
Purity: > 80%
Traits: Freeze-dried powder
Buffer formulation: 100mMNaHCO3, 500mMNaCl, pH8.3, containing 0.01% SKL, 5%Trehalose.
Original Concentration: 200ug/mL
Applications: Positive Control; Immunogen; SDS-PAGE; WB.
(May be suitable for use in other assays to be determined by the end user.)
Predicted isoelectric point: 7.7
Predicted Molecular Mass:37.4kDa
Accurate Molecular Mass: 41kDa as determined by SDS-PAGE reducing conditions.
Phenomenon explanation:
The possible reasons that the actual band size differs from the predicted are as follows:
1.Splice variants: Alternative splicing may create different sized proteins from the same gene.
2. Relative charge: The composition of amino acids may affects the charge of the protein.
3. Post-translational modification: Phosphorylation, glycosylation, methylation etc.
4. Post-translation cleavage: Many proteins are synthesized as pro-proteins, and then cleavedto give the active form.
5. Polymerization of the target protein: Dimerization, multimerization etc.
[ USAGE ]
Reconstitute in 100mM NaHCO3, 500mM NaCl (pH8.3) to a concentration of 0.1-1.0 mg/mL. Donot vortex.
[ STORAGE AND STABILITY ]
Storage: Avoid repeated freeze/thaw cycles.
Store at 2-8°Cfor one month.
Aliquot and store at -80°Cfor 12 months.
Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
[SEQUENCE ]
[ IDENTIFICATION ]
[ IMPORTANT NOTE ]
The kit is designed for research use only, we will not be responsible for any issue if the kit was used in clinical diagnostic or any other procedures.
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關(guān)鍵詞:MIR16 Membrane Interacting Protein Of RGS16 RGS16-interacting membrane protein