尚寶生物 deae-dextran細(xì)胞轉(zhuǎn)染試劑盒(deae-dextran cell transfection kit)適用于瞬時(shí)轉(zhuǎn)染 (transient tranfection) ,不宜用于篩選穩(wěn)定表達(dá)細(xì)胞株,這是因?yàn)閐eae-dextran在較高濃度作用較長(zhǎng)時(shí)間會(huì)對(duì)細(xì)胞產(chǎn)生一定毒性。氯喹的加入可以抑制溶酶體對(duì)外源dna的降解,從而提高轉(zhuǎn)染效率。但對(duì)于具體的細(xì)胞、具體的培養(yǎng)條件,如需獲得更加理
更新時(shí)間:2025-01-07